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ОБНБиохимия Biochemistry

  • ISSN (Print) 0320-9725
  • ISSN (Online) 3034-5294

СВЕРХСПИРАЛИЗАЦИЯ ДНК СНИЖАЕТ ХОЛОДОЧУВСТВИТЕЛЬНОСТЬ ПЛАВЛЕНИЯ ПРОМОТОРА РНК-ПОЛИМЕРАЗАМИ Deinococcus-Thermus

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Код статьи
S30345294S0320972525080099-1
DOI
10.7868/S3034529425080099
Тип публикации
Статья
Статус публикации
Опубликовано
Авторы
Кульбачинский А.В.
  • Аффилиация:
    НИЦ «Курчатовский Институт»
    Институт биологии гена Российской академии наук
Том/ Выпуск
Том 90 / Номер выпуска 8
Страницы
1189-1200
Аннотация
Плавление промоторной ДНК вокруг стартовой точки транскрипции является важнейшим этапом транскрипции, необходимым для инициации синтеза РНК. У бактерий плавление промотора осуществляется холоферментом РНК-полимеразы (РНКП), состоящим из каталитического кор-фермента и σ-фактора, участвующего в распознавании промотора. Ранее было обнаружено, что РНКП из термофильной бактерии и мезофильной бактерии не способны инициировать транскрипцию при низкой или умеренной температуре. Эти свойства зависят от их σ-факторов, а гибридные холоферменты, включающие эти σ-факторы и кор-фермент , обладают похожими свойствами. В данной работе показано, что сверхспирализация ДНК снижает холодочувствительность плавления промотора РНКП Deinococcus-Thermus и гибридными холоферментами. Сверхспирализация также повышает стабильность промоторных комплексов этих РНКП и снижает их чувствительность к высокой ионной силе. Подобные свойства РНКП Deinococcus-Thermus позволяют предположить, что их активность может регулироваться степенью сверхспирализации генома, в том числе при стрессовом ответе клеток.
Ключевые слова
РНК-полимераза Deinococcus radiodurans сверхспирализация ДНК плавление промотора устойчивость к стрессу
Дата публикации
17.06.2025
Год выхода
2025
Всего подписок
0
Всего просмотров
77

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