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RAS BiologyБиохимия Biochemistry

  • ISSN (Print) 0320-9725
  • ISSN (Online) 3034-5294

EFFECT OF CULTIVATION CONDITIONS ON THE EXPRESSION OF THE Exiguobacterium sibiricum PROTEORHODOPSIN GENE

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PII
S30345294S0320972525070088-1
DOI
10.7868/S3034529425070088
Publication type
Article
Status
Published
Authors
L.E. Petrovskaya
  • Affiliation:
    Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
    Moscow Institute of Physics and Technology
E.V. Spirina
  • Affiliation: Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences
A.Yu. Sukhanov
  • Affiliation: FRC Kazan Scientific Center, Russian Academy of Sciences
E.A. Kryukova
  • Affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
E.P. Lukashev
  • Affiliation:
    Lomonosov Moscow State University
    Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
R.H. Ziganshin
  • Affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
E.M. Rivkina
  • Affiliation: Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences
D.A. Dolgikh
  • Affiliation:
    Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
    Lomonosov Moscow State University
M.P. Kirpichnikov
  • Affiliation:
    Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
    Lomonosov Moscow State University
Volume/ Edition
Volume 90 / Issue number 7
Pages
993-1003
Abstract
Recombinant proteorhodopsin ESR of the gram-positive bacterium Exiguobacterium sibiricum, isolated from permafrost deposits in northeastern Siberia, binds retinal and is a light-dependent proton pump, but nothing is known about its expression in natural conditions. In this work, expression of the esr gene in cultures of E. sibiricum grown under various conditions was studied by quantitative PCR. It has been established that cultivation on poor media at low temperatures contributes to a significant increase in the synthesis level of the corresponding mRNA. The data obtained are confirmed by the results of the analysis of the cells' membrane fraction using mass spectrometry. Also, at 10 °C, increased content of phytocandesaturases, which are involved in the biosynthesis of carotenoids, is observed. However, we were unable to detect the presence of a functional retinal-containing protein in the cells, presumably due to the lack of an enzymatic retinal synthesis system in E. sibiricum. The possible functions of ESR in E. sibiricum cells are discussed in connection with the characteristics of the bacterium's extreme habitat. The results of the work contribute to understanding of the molecular mechanisms of microbial adaptation to environmental conditions and the potential role of microbial rhodopsin in these processes.
Keywords
Exiguobacterium sibiricum многолетнемерзлые отложения микробные родопсины ретиналь каротиноиды
Date of publication
04.02.2026
Year of publication
2026
Number of purchasers
0
Views
83

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