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RAS BiologyБиохимия Biochemistry

  • ISSN (Print) 0320-9725
  • ISSN (Online) 3034-5294

GLYCEROL KINASE OVEREXPRESSION SUPRESSES LIPID SYNTHESIS BUT ENLARGES MITOCHONDRIAL MEMBRANE POTENTIAL AND THERMOGENESIS ACTIVITY IN ADIPOCYTES

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PII
S30345294S0320972525050079-1
DOI
10.7868/S3034529425050079
Publication type
Article
Status
Published
Authors
S. S. Michurina
  • Affiliation: National Medical Research Centre for Cardiology Named after Academician E.I. Chazov
I. B. Beloglazova
  • Affiliation: National Medical Research Centre for Cardiology Named after Academician E.I. Chazov
M. Yu. Agareva
  • Affiliation:
    National Medical Research Centre for Cardiology Named after Academician E.I. Chazov
    Faculty of Basic Medicine, Lomonosov Moscow State University
R. Mohammad
  • Affiliation: Moscow Institute of Physics and Technology (National Research University)
N. V. Alekseeva
  • Affiliation: Faculty of Biology, Lomonosov Moscow State University
Ye. V. Parfyonova
  • Affiliation:
    National Medical Research Centre for Cardiology Named after Academician E.I. Chazov
    Faculty of Basic Medicine, Lomonosov Moscow State University
I. S. Stafeev
  • Affiliation: National Medical Research Centre for Cardiology Named after Academician E.I. Chazov
Volume/ Edition
Volume 90 / Issue number 5
Pages
673-687
Abstract
Obesity and type 2 diabetes mellitus are among the main factors contributing to the increase in mortality and disability in the modern world. Therefore, it is a priority to develop new methods, including genetic and cellular engineering, to create ectopic thermogenic fat depots capable of dissipating excess energy. In this study, we overexpressed glycerol kinase (GK), a key enzyme of the futile triacylglyceride cycle (TAG cycle) to generate thermogenic adipocytes. The protein-coding sequence of GK was amplified from mouse liver mRNA and delivered to adipocytes by lentiviral transduction. Adipocyte metabolism was analyzed by radioisotope monitoring of [3H]- and [14C]-labelled glucose analogues. Mitochondrial membrane potential, thermogenesis and lipid droplet morphology were assessed using fluorescent probes JC-1, ERthermAC and BODIPY493/503, respectively. Lentiviral delivery of the GK gene increases mRNA expression 130-fold and protein levels by 30% in adipocytes. GK overexpression enhances glucose uptake by adipocytes and suppresses fatty acids synthesis and re-esterification without altering lipid droplet morphology. The increase in glucose uptake upon GK overexpression is associated with an increase in mitochondrial potential and stimulation of thermogenesis. GK overexpression improves the metabolic profile of adipocytes, which may contribute to the elimination of metabolic disorders associated with obesity by increasing the utilization of excess glucose during thermogenesis. Nevertheless, the detailed mechanisms underlying the stimulation of these processes require further investigation.
Keywords
глицеролкиназа адипоциты термогенез метаболизм глюкозы метаболизм липидов
Date of publication
04.02.2026
Year of publication
2026
Number of purchasers
0
Views
89

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