- PII
- S30345294S0320972525050037-1
- DOI
- 10.7868/S3034529425050037
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 90 / Issue number 5
- Pages
- 627-635
- Abstract
- Changes in the Na+ and K+ intracellular concentrations affect expression of the FOS gene. Here, we obtained a genetic construct coding for the TurboGFP-dest1 protein under control of the human FOS promoter (-549 to +155) and studied its expression in HEK293T cells exposed to monovalent metal cations. Amplification of the FOS promoter sequence from genomic DNA was efficient only in the presence of Li+ ions. Incubation of cells with ouabain or in a medium containing Li+ ions instead of Na+ ions caused intracellular accumulation of Na+ and Li+ ions, respectively. In addition, both stimuli increased the levels of endogenous FOS mRNA and the average fluorescence intensity of TurboGFP-dest1 in transfected cells. The mRNA levels of TurboGFP-dest1 were significantly higher than the FOS mRNA levels and were little affected by the stimuli.
- Keywords
- литий натрий уабаин FOS транскрипция
- Date of publication
- 04.02.2026
- Year of publication
- 2026
- Number of purchasers
- 0
- Views
- 90
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