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RAS BiologyБиохимия Biochemistry

  • ISSN (Print) 0320-9725
  • ISSN (Online) 3034-5294

CATIONIC ANTISEPTICS DISRUPT THE FUNCTIONING OF THE ELECTRON-TRANSPORT CHAIN AT THE ACCEPTOR SITE IN THE PHOTOSYNTHETIC REACTION CENTRES OF THE PURPLE BACTERIUM Cereibacter sphaeroides

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PII
S30345294S0320972525070067-1
DOI
10.7868/S3034529425070067
Publication type
Article
Status
Published
Authors
E.P. Lukashev
  • Affiliation: Department of Biophysics, Faculty of Biology, Lomonosov Moscow State University
M.D. Mamedov
  • Affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University
L.A. Vitukhnovskya
  • Affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University
A.M. Mamedova
  • Affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University
P.P. Knox
  • Affiliation: Department of Biophysics, Faculty of Biology, Lomonosov Moscow State University
V.Z. Paschenko
  • Affiliation: Department of Biophysics, Faculty of Biology, Lomonosov Moscow State University
Volume/ Edition
Volume 90 / Issue number 7
Pages
961-973
Abstract
Electrometric technique revealed that the cationic antiseptic octenidine reduces the generation of transmembrane electrical potential difference in the chromatophores of photosynthetic bacterium Cereibacter sphaeroides. This is also confirmed by measurements of electrochromic shifts of carotenoid absorption bands in chromatophores. In reaction centers (RCs) isolated from chromatophores in the absence of external electron donors and acceptors, the rate of recombination between photooxidized bacteriochlorophyll P and reduced secondary quinone acceptor Q, as measured by absorption changes in the near infrared region, was very weakly dependent on the presence of antiseptics, in contrast to the kinetics in the 400-600 nm spectral range, where absorption changes associated with the oxidation of P and the formation of semiquinone radicals Q and Q, as well as electrochromic shifts of the carotenoid and bacteriopheophytin RC absorption bands are observed. The addition of cationic antiseptics resulted in the appearance of absorption changes in this region with the formation time of about 100-200 ms and a decay time of about 3 s. In the series: picoxidine – chlorhexidine – octenidine – miramistin, the latter was the most effective. The maximum amplitude of such changes was observed in the absorption region of the semiquinone radical around 460 nm. When blocking the electron transfer from Q to Q by o-phenanthroline, the effect disappeared. It is suggested that cationic antiseptics stimulate protonation of Q with the formation of a neutral QH complex.
Keywords
катионные антисептики хроматофоры реакционные центры электрогенез электрометрический метод флеш-фотолиз
Date of publication
04.02.2026
Year of publication
2026
Number of purchasers
0
Views
97

References

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