- PII
- S30345294S0320972525040061-1
- DOI
- 10.7868/S3034529425040061
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 90 / Issue number 4
- Pages
- 571-579
- Abstract
- The structural and functional issues of protein functionality of restriction-modification systems recognizing one of the GGATC/GATCC, GATGC/GCATC, and GATGG/CCATC sites have been studied using bioinformatics methods. Such systems include a single restriction endonuclease and either two separate DNA methyltransferases or a single fusion DNA methyltransferase with two catalytic domains. For a subset of these systems, it was known that both adenines within the site are methylated to form 6-methyladenine, but the role of each of the two DNA methyltransferases comprising the system was unknown. In this work, the functionality of most known systems of this kind is proven. Based on analysis of the structures of related DNA methyltransferases, it is hypothesized which of the adenines within the site is modified by each of the DNA methyltransferases of the system. A possible molecular mechanism of DNA methyltransferase specificity change from GATGG to GATGC during horizontal transfer of its gene is described.
- Keywords
- система рестрикции-модификации пространственная структура ДНК-метилтрансфераза эндонуклеаза рестрикции горизонтальный перенос генов
- Date of publication
- 25.03.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 77
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